Difference between revisions of "Part:BBa K1465204:Design"

Revision as of 00:18, 2 October 2014

Shell-associated protein (csoS2) of the carboxysome of Halothiobacillus neapolitanus

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 185
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 343
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 851
Illegal AgeI site found at 1802
Illegal AgeI site found at 2483
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 243

Design Notes

Gensynthesis (gBlocks) and PCR-amplification

Source

[http://www.ncbi.nlm.nih.gov/nucleotide/261835099?report=genbank&log$=nuclalign&blast_rank=1&RID=2T27D737014 Halothiobacillus neapolitanus c2 (GenBank: CP001801.1)]: Hneap 0920

[http://www.addgene.org/52065/ Plasmid] from Bonacci et al, 2011

References

Walter Bonacci, Poh K. Teng, Bruno Afonso, Henrike Niederholtmeyer, Patricia Grob, Pamela A. Silver

and David F. Savage (2011) Modularity of a carbon-fixing protein organelle [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258634/pdf/pnas.201108557.pdf Proc Natl Acad Sci U S A. 109: 478–483.]

@@ Line 13: / Line 13: @@
 ===Source===
-Gibson-Assembly
+* [http://www.ncbi.nlm.nih.gov/nucleotide/261835099?report=genbank&log$=nuclalign&blast_rank=1&RID=2T27D737014 ''Halothiobacillus neapolitanus c2'' (GenBank: CP001801.1)]: Hneap 0920
+* [http://www.addgene.org/52065/ Plasmid] from Bonacci ''et al'', 2011
 ===References===