Difference between revisions of "Part:BBa K1465207:Design"

(→‎Design Notes)
(→‎Design Notes)
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  5'-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3'<br>
 
  5'-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3'<br>
  
−
*Amplification of the csoS1D containing also the natural interspace with the RBS using specific the Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#csoS1D_rev csoS1D_rev] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#csoS1D_fwd csoS1D_fwd]:<br>
+
*Amplification of the csoS1D containing also the natural interspace with the RBS using specific the Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#csoS1D_fwd csoS1D_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#csoS1D_rev csoS1D_rev]:<br>
  
−
Primer
+
'''csoS1D_fwd (40 bp)'''<br>
 +
5'-ATTCGCGGCCGCTTCTAGAGTGTCTAGTAGGGAAGATGCG-3'<br>
 +
'''csoS1D_rev (41 bp)'''<br>
 +
5'-TGCAGCGGCCGCTACTAGTATTAGAACCCTTCAGCGCGACG-3'<br>
  
−
*[http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the optimized coding sequence of the Phosphoribulokinase (''prkA'') from ''Synechococcus elangatus''.<br>
+
*[http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the coding sequence of csoS!D containing also the natural interspace region with RBS from ''Halothiobacillus neapolitanus''.<br>
  
 
===Source===
 
===Source===

Revision as of 22:44, 1 October 2014


Pore-forming protein (csoS1D) of the carboxysome of Halothiobacillus neapolitanus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Amplification of the pSB1C3-Backbone using specific the Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_csoS1D pSB1C3_pre_csoS1D] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_csoS1D pSB1C3_suf_csoS1D]:
pSB1C3_pre_csoS1D (43 bp)

5'-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_csoS1D (43 bp)

5'-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3'
  • Amplification of the csoS1D containing also the natural interspace with the RBS using specific the Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#csoS1D_fwd csoS1D_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#csoS1D_rev csoS1D_rev]:
csoS1D_fwd (40 bp)

5'-ATTCGCGGCCGCTTCTAGAGTGTCTAGTAGGGAAGATGCG-3'

csoS1D_rev (41 bp)

5'-TGCAGCGGCCGCTACTAGTATTAGAACCCTTCAGCGCGACG-3'
  • [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the coding sequence of csoS!D containing also the natural interspace region with RBS from Halothiobacillus neapolitanus.

Source

PCR

References