Difference between revisions of "Part:BBa K1470001"
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This enzyme is often used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis nearly over 99 % of uninfected cells will be dead within two days.
 
This enzyme is often used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis nearly over 99 % of uninfected cells will be dead within two days.
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References:
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PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS., DARKEN MA.,Pharmacol Rev. 1964 Sep;16:223-43., PMID:14211122
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NEUBERT, D. AND LEHNINGER, A. L.: The effect of oligomycin, gramicidin, and other antibiotics on reversal of
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mitochondrial swelling by adenosine triphosphate. Biochim. biophys. Acta 62: 556-565, 1962., http://dx.doi.org/10.1016/0006-3002(62)90238-X, http://www.sciencedirect.com/science/article/pii/000630026290238X
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:41, 1 October 2014

puromycin N-acetyl-transferase

Puromycin is an antibodic derived from Streptomyces alboniger. The substance affects the cell both in protein translation and mitochondrial protein import. Due to this, it's toxic to many organisms such as bacteria, Trypanosoma and humans.

A small part of its structure resembles to aminoacetylated tRNA and is recognized by ribosomes. the nascent chain containing puromycin leads to a translational stop.

Streptomyces alboniger protects itself from puromycin with a acetyl transferase. It uses acetyl-CoA to bind the the acetyl group with the amino group. In this state puromycin cannot be recognized by the ribosom anymore.

This enzyme is often used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis nearly over 99 % of uninfected cells will be dead within two days.

References:

PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS., DARKEN MA.,Pharmacol Rev. 1964 Sep;16:223-43., PMID:14211122

NEUBERT, D. AND LEHNINGER, A. L.: The effect of oligomycin, gramicidin, and other antibiotics on reversal of mitochondrial swelling by adenosine triphosphate. Biochim. biophys. Acta 62: 556-565, 1962., http://dx.doi.org/10.1016/0006-3002(62)90238-X, http://www.sciencedirect.com/science/article/pii/000630026290238X

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 481