Difference between revisions of "Part:BBa K1470001"

Revision as of 13:23, 1 October 2014

puromycin N-acetyl-transferase

Puromycin is an antibodic derived from Streptomyces alboniger. The substance affects the cell both in protein translation and mitochondrial protein import. Due to this, it's toxic to many organisms such as bacteria, Trypanosoma and humans.

A small part of its structure resembles to aminoacetylated tRNA and is recognized by ribosomes. the nascent chain containing puromycin leads to a translational stop.

Streptomyces alboniger protects itself from puromycin with a acetyl transferase. It uses acetyl-CoA to bind the the acetyl group with the amino group. In this state puromycin cannot be recognized by the ribosom anymore.

This enzyme is often used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis nearly over 99 % of uninfected cells will be dead within two days.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 481

@@ Line 8: / Line 8: @@
 Streptomyces alboniger protects itself from puromycin with a acetyl transferase. It uses acetyl-CoA to bind the the acetyl group with the amino group. In this state puromycin cannot be recognized by the ribosom anymore.
-This enzyme is often used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis nearly over 99 % of uninfected cells will be dead within tows days.
+This enzyme is often used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis nearly over 99 % of uninfected cells will be dead within two days.
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