Difference between revisions of "Part:BBa K1365006:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
Used primers:  
 
Used primers:  
−
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTATAAAATTTTAATAGT-3'  
+
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTATAAAATTTTAATAGT-3' </b>
−
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA-3'  
+
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA-3' </b>
−
to PCR NisR and NisK from the genome and add the BioBrick prefix and suffix.  
+
to PCR NisR and NisK from the genome and add the BioBrick prefix and suffix. </b></b>
  
 
Changed the GTG start codon of NisR into a ATG.
 
Changed the GTG start codon of NisR into a ATG.
−
 
−
 
−
 
  
 
===Source===
 
===Source===

Revision as of 07:54, 30 September 2014


NisR and NisK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used primers: forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTATAAAATTTTAATAGT-3' </b> reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA-3' </b> to PCR NisR and NisK from the genome and add the BioBrick prefix and suffix. </b></b>

Changed the GTG start codon of NisR into a ATG.

Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

References