Difference between revisions of "Part:BBa F2620"

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|width="240px"|<span style="font-family: Arial Black; font-size: 18pt">BBa_F2620</span><br>
 
|width="240px"|<span style="font-family: Arial Black; font-size: 18pt">BBa_F2620</span><br>
  
  
 
<span style="font-family: Arial; font-size: 12pt">3OC<sub>6</sub>HSL -> PoPS Receiver</span><br>
 
<span style="font-family: Arial; font-size: 12pt">3OC<sub>6</sub>HSL -> PoPS Receiver</span><br>
|width="240px" align="center"|[[Image:F2620.jpg|200px]]
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|width="240px" align="center"|<html><DIV><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;'>tetR<br>R0040</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>LuxR<br>I0462</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>lux pR<br>R0062</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
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|width="240px" align="center" valign="bottom"|<html><DIV><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;'>tetR<br>R0040</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>LuxR<br>I0462</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>lux pR<br>R0062</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
 
promoter (tetR, negative)' src='/r/parts/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462
 
promoter (tetR, negative)' src='/r/parts/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462
 
luxR Protein Generator' src='/r/parts/images/partbypart/icon_generator.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=192'><img title='BBa_R0062
 
luxR Protein Generator' src='/r/parts/images/partbypart/icon_generator.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=192'><img title='BBa_R0062

Revision as of 23:15, 17 September 2006

A work in progress...

BBa_F2620


3OC6HSL -> PoPS Receiver

F2620.jpg
tetR
R0040
LuxR
I0462
lux pR
R0062


Authors: Barry Canton [bcanton@mit.edu], Anna Labno [labnoa@mit.edu]                                     Last Update: Sept. 15th, 2006


Description
A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by an operator [TetR]. Device input is 3OC6HSL. Device output is PoPS produced at a LuxR-regulated operator.

Usage
Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics
Input Swing: ## nM 3OC6HSL, exogenous
Output Swing: #-# PoPS
Switch Point: 2 nM 3OC6HSL, exogenous
L/H Latency: # minutes
Key Subparts
BBa_R0040: TetR-regulated operator
BBa_C0062: luxR ORF
BBa_R0062: LuxR-regulated operator


Transfer Function
BC-TransferCurve-3.png
Specificity
BC-Specificity-1.png
Latency
BC-Latency-2.png
Stability
BC-Stability-1.png


Demand
Transcription Demand: ## NTP per second
## polymerases sequestered
Translation Demand: # charged tRNA per second
# ribosomes sequestered
Stability
Genetic Stability: ##/## replication events (Lo Input/High Input)
Performance Stability: ##/## replication events (Lo Input/High Input)

Compatibility
Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Device has been shown to work on pSB3K3 and pSB3K3
Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
All characterization was performed on pSB3K3 using MG1655 as the characterization chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.


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