Difference between revisions of "Part:BBa F2620"
Line 2: | Line 2: | ||
<div style="padding: 40px; width: 720px; border: 1px solid #000000;"> | <div style="padding: 40px; width: 720px; border: 1px solid #000000;"> | ||
− | {| | + | {| |
|width="240px"|<span style="font-family: Arial Black; font-size: 18pt">BBa_F2620</span><br> | |width="240px"|<span style="font-family: Arial Black; font-size: 18pt">BBa_F2620</span><br> | ||
<span style="font-family: Arial; font-size: 12pt">3OC<sub>6</sub>HSL -> PoPS Receiver</span><br> | <span style="font-family: Arial; font-size: 12pt">3OC<sub>6</sub>HSL -> PoPS Receiver</span><br> | ||
− | |width="240px" align="center"|[[Image:F2620.jpg|200px]] | + | |width="240px" align="center" valign="top"|[[Image:F2620.jpg|200px]] |
− | |width="240px" align="center"|<html><DIV><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;'>tetR<br>R0040</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>LuxR<br>I0462</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>lux pR<br>R0062</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040 | + | |width="240px" align="center" valign="bottom"|<html><DIV><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;'>tetR<br>R0040</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>LuxR<br>I0462</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>lux pR<br>R0062</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040 |
promoter (tetR, negative)' src='/r/parts/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462 | promoter (tetR, negative)' src='/r/parts/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462 | ||
luxR Protein Generator' src='/r/parts/images/partbypart/icon_generator.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=192'><img title='BBa_R0062 | luxR Protein Generator' src='/r/parts/images/partbypart/icon_generator.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=192'><img title='BBa_R0062 |
Revision as of 23:15, 17 September 2006
A work in progress...
BBa_F2620
|
|
Authors: Barry Canton [bcanton@mit.edu], Anna Labno [labnoa@mit.edu] Last Update: Sept. 15th, 2006
Description
A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by an operator [TetR]. Device input is 3OC6HSL. Device output is PoPS produced at a LuxR-regulated operator.
Usage
Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
Characteristics
|
Key Subparts
|
Transfer Function |
Specificity |
Latency |
Stability |
Demand
|
Stability
|
Compatibility
Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Device has been shown to work on pSB3K3 and pSB3K3
Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here.
Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
All characterization was performed on pSB3K3 using MG1655 as the characterization chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.