Difference between revisions of "Part:BBa K1439000:Design"

(Source)
(Design Notes)
 
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===Design Notes===
 
===Design Notes===
OriTRP4 region contains some promoters,we put it in the back of terminator.In order to reduce the impact on protein expression.
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OriTRP4 region contains some promoters, we put it in the back of terminator.In order to reduce the impact on protein expression.
 
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+
  
 
===Source===
 
===Source===

Latest revision as of 01:48, 30 September 2014


Origin of transfer for the RP4-plasmid nic region.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 20
    Illegal NgoMIV site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

OriTRP4 region contains some promoters, we put it in the back of terminator.In order to reduce the impact on protein expression.

Source

This part comes form the RP4 plasmid, from 51098bp to 51448bp.

References