Difference between revisions of "Part:BBa K1465203:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
  
−
Gibson_assembly
+
*Amplification of the pSB1C3-Backbone using specific the Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_rbcLS pSB1C3_pre_rbcLS] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_rbcLS pSB1C3_suf_rbcLS]:<br>
 +
 
 +
'''pSB1C3_pre_rbcLS (44 bp)'''<br>
 +
5'-CATCTAGCGGCCGCTACTAGTATTAGATCTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'<br>
 +
'''pSB1C3_suf_rbcLS (43 bp)'''<br>
 +
5'-TGGTTTACCGCGGCAACTAATACTAGTAGCGGCCGCTGCAGTC-3'<br>
 +
 
 +
*[http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the optimized coding sequence of the Ribulose-1,5-bisphosphate carboxylase/oxygenase (''rbcLS'') from ''Synechococcus elangatus''.<br>
  
 
===Source===
 
===Source===

Revision as of 22:45, 29 September 2014


Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Synechococcus elongatus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1177
    Illegal NgoMIV site found at 1524
    Illegal AgeI site found at 550
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Amplification of the pSB1C3-Backbone using specific the Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_rbcLS pSB1C3_pre_rbcLS] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_rbcLS pSB1C3_suf_rbcLS]:
pSB1C3_pre_rbcLS (44 bp)

5'-CATCTAGCGGCCGCTACTAGTATTAGATCTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_rbcLS (43 bp)

5'-TGGTTTACCGCGGCAACTAATACTAGTAGCGGCCGCTGCAGTC-3'
  • [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the optimized coding sequence of the Ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcLS) from Synechococcus elangatus.

Source

Gensynthesis (gBlocks)

References