Difference between revisions of "Part:BBa K1554004:Design"
(→Design Notes) |
|||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | The assembly was performed by GoldenBraid assembly. The barnase was | + | The assembly was performed by GoldenBraid 2.0 assembly. The barnase was amplified by PCR from [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] and a mutation in T105P was introduced. |
− | + | ||
− | + | ||
===Source=== | ===Source=== |
Revision as of 15:43, 29 September 2014
Yellow biosafety module for plants
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The assembly was performed by GoldenBraid 2.0 assembly. The barnase was amplified by PCR from Part:BBa_J72173 and a mutation in T105P was introduced.
Source
TA29 promoter Part:BBa_K1554000
Barnase Part:BBa_J72173
AmilGFP [Waiting for Norwich team part name in registry]
P35s Part:BBa_K1537015