Difference between revisions of "Part:BBa F2620"
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|align="left"|'''Latency'''<br>[[Image:BC-Latency-2.png|300px]]
 
|align="left"|'''Latency'''<br>[[Image:BC-Latency-2.png|300px]]
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|align="right"|'''Stability'''<br>[[Image:BC-Stability-1.png|225px]]
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|align="right"|'''Stability'''<br>[[Image:BC-Stability-1.png|300px]]
 
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Device has been shown to work with ''<partinfo>BBa_E0430</partinfo>'',''<partinfo>BBa_E0434</partinfo>'',''<partinfo>BBa_E0240</partinfo>''<br>
 
Device has been shown to work with ''<partinfo>BBa_E0430</partinfo>'',''<partinfo>BBa_E0434</partinfo>'',''<partinfo>BBa_E0240</partinfo>''<br>
 
Crosstalk with devices using 3OC<sub>6</sub>HSL or add in the other molecule names here.
 
Crosstalk with devices using 3OC<sub>6</sub>HSL or add in the other molecule names here.
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Crosstalk with systems containing ''TetR'' (''<partinfo>BBa_C0040</partinfo>'')
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Crosstalk with systems containing ''TetR'' (''<partinfo>BBa_C0040</partinfo>'')<br>
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'''Characterization Conditions'''<br>
 
</div>
 
</div>
  
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<partinfo>BBa_F2620 short</partinfo>
 
 
<partinfo>BBa_F2620 SpecifiedComponents</partinfo>
 
<partinfo>BBa_F2620 SpecifiedComponents</partinfo>
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_F2620 SequenceAndFeatures</partinfo>
 
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===Device Characteristics===
 
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<partinfo>BBa_F2620 parameters</partinfo>
 
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Get the device [http://openwetware.org/wiki/Image:F2620.de2-ania.ppt#filelinks datasheet]
 
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====Transfer Function====
 
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====Latency====
 
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====Specificity====
 
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[http://openwetware.org/images/9/93/Transfer.jpg Specificity preliminary data]
 
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====Stability====
 
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[http://openwetware.org/images/b/b9/StabilitySlideCropped.jpg Stability preliminary data]
 

Revision as of 00:50, 16 September 2006

A work in progress...

BBa_F2620


3OC6HSL -> PoPS Receiver

 F2620.jpg

Authors: Barry Canton [bcanton@mit.edu], Anna Labno [labnoa@mit.edu]                                     Last Update: Sept. 15th, 2006

Description
A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by an operator [TetR]. Device input is 3OC6HSL. Device output is PoPS produced at a LuxR-regulated operator.

Usage
Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., Part:pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics
Input Swing: ## nM 3OC6HSL, exogenous
Output Swing: #-# PoPS
Switch Point: 2 nM 3OC6HSL, exogenous
L/H Latency: # minutes
Key Subparts
BBa_R0040: TetR-regulated operator
BBa_C0062: luxR ORF
BBa_R0062: LuxR-regulated operator


Transfer Function
BC-TransferCurve-3.png 		Specificity
BC-Specificity-1.png
Latency
BC-Latency-2.png 		Stability
BC-Stability-1.png


Demand
Transcription Demand: ## NTP per second
## polymerases sequestered
Translation Demand: # charged tRNA per second
# ribosomes sequestered
Stability
Genetic Stability: ##/## replication events (Lo Input/High Input)
Performance Stability: ##/## replication events (Lo Input/High Input)

Compatibility
Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Device has been shown to work on pSB3K3 and pSB3K3
Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions


RoSBP Banner.jpg


p(tetR)
R0040
B0034		luxr
C0062
B0015		lux pR
R0062