Difference between revisions of "Part:BBa S03595:Experience"

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===Applications of BBa_S03595===
 
===Applications of BBa_S03595===
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This part was constructed to test the capacity of the double forward terminator BBa_B0015 to block read-through transcription.  
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This part was constructed to test the capacity of the Double Forward Terminator [[https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]] to block read-through transcription.  
  
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Part [[https://parts.igem.org/wiki/index.php/Part:BBa_S03562 BBa_S03562]] which contains TetR with a ribosomal binding site is sufficient to convey tetracycline resistance, although there is no promoter. We suspected that TetR might be expressed via read-through transcription from the carrier vector.
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Part [[https://parts.igem.org/wiki/index.php/Part:BBa_S03562 BBa_S03562]], which contains TetR with a ribosomal binding site, is sufficient to convey tetracycline resistance in the absence of a promoter. We suspected that TetR might be expressed via read-through transcription from the carrier vector.
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Consistent with this observation, the following parts are also expressed in the absence of an upstream promoter:
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* Insert list here
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Once the Double Forward Terminator [[https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]] is placed upstream of RBS-TetR, the cells are no longer tetracycline resistant. We conclude that the Double Forward Terminator is sufficient to block read-through transcription, allowing control over the expression of the downstream coding region.
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Construction of an "insulator vector" where the double terminator is placed before the BioBrick prefix and after the BioBrick suffix (in the reverse orientation) is currently underweigh.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 22:09, 14 September 2006


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_S03595

This part was constructed to test the capacity of the Double Forward Terminator [BBa_B0015] to block read-through transcription.

Part [BBa_S03562], which contains TetR with a ribosomal binding site, is sufficient to convey tetracycline resistance in the absence of a promoter. We suspected that TetR might be expressed via read-through transcription from the carrier vector.

Consistent with this observation, the following parts are also expressed in the absence of an upstream promoter:

  • Insert list here

Once the Double Forward Terminator [BBa_B0015] is placed upstream of RBS-TetR, the cells are no longer tetracycline resistant. We conclude that the Double Forward Terminator is sufficient to block read-through transcription, allowing control over the expression of the downstream coding region.

Construction of an "insulator vector" where the double terminator is placed before the BioBrick prefix and after the BioBrick suffix (in the reverse orientation) is currently underweigh.

User Reviews

UNIQe3d7071932f780b3-partinfo-00000000-QINU UNIQe3d7071932f780b3-partinfo-00000001-QINU