Difference between revisions of "Part:BBa K1554001"

Revision as of 15:46, 28 September 2014

HarFAR

Delta 11 desaturase from Amyelois transitella. Codon optimization was perfomed for Nicotiana benthamiana.

Usage and Biology

The AtrDelta11 protein is a delta 11 desaturase coming from Amyelois transitella which introduces a desaturation in fatty acids at the 11th bond.

acyl-CoA + reduced acceptor + O2 = Delta11-acyl-CoA + acceptor + 2 H2O

Our team used it in with Part:BBa_K1554001 to produce the insect pheromone Z11-16:OH in Nicotiana benthamiana using palmitate:CoA as precursor. It was also used with Part:BBa_K1554003 to transform Z11-16:OH into antoher insect pheromone, Z11-16:OAc.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1104
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 929

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1554002 short</partinfo>
-Delta11 desaturase
+Delta 11 desaturase from ''Amyelois transitella''. Codon optimization was perfomed for ''Nicotiana benthamiana''.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+The AtrDelta11 protein is a delta 11 desaturase coming from ''Amyelois transitella'' which introduces a desaturation in fatty acids at the 11th bond.
+acyl-CoA + reduced acceptor + O2 = Delta11-acyl-CoA + acceptor + 2 H2O
+Our team used it in with [https://parts.igem.org/Part:BBa_K1554001 Part:BBa_K1554001] to produce the insect pheromone Z11-16:OH in Nicotiana benthamiana using palmitate:CoA as precursor. It was also used with Part:BBa_K1554003 to transform Z11-16:OH into antoher insect pheromone, Z11-16:OAc.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1554002 SequenceAndFeatures</partinfo>