Difference between revisions of "Part:BBa K1486024"

Yeast kanamycin resistance

Purpose of the Biobrick

This part encodes for the KanMx resistance gene and confers dominant selection of geneticin to yeast. We fused it to our constructs to select the transformants after homologous recombinations. It was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3.

Experiment 1:

Associated Biobricks

This part was received with the terminator ADH1 and we added it to all our constructs being fused with the pbs2 gene.

• yeast-sfGFP-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486026)
• RLuc-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486027)
• yeast-sfGFP_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486029)
• RLuc_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486030)