Difference between revisions of "Part:BBa K1486024"
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__NOTOC__ | __NOTOC__ | ||
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+ | <partinfo>BBa_K1486023 short</partinfo> | ||
+ | </P></FONT> | ||
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+ | <FONT SIZE="3"><P> | ||
+ | ===Purpose of the Biobrick=== | ||
+ | </P></FONT> | ||
+ | |||
+ | <FONT SIZE="2"><P> | ||
+ | This part encodes for the KanMx resistance gene and confers dominant selection of geneticin to yeast. We fused it to our constructs to select the transformants after homologous recombinations. It was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3. | ||
+ | |||
+ | </P></FONT> | ||
+ | |||
+ | <FONT SIZE="2"><P> | ||
+ | |||
+ | </P></FONT> | ||
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+ | <FONT SIZE="2"><P> | ||
+ | |||
+ | </P></FONT> | ||
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+ | |||
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+ | <FONT SIZE="3"><P> | ||
+ | |||
+ | ===Experiment 1:=== | ||
+ | </P></FONT> | ||
+ | |||
+ | <FONT SIZE="2"><P> | ||
+ | |||
+ | ===Associated Biobricks=== | ||
+ | </P></FONT> | ||
+ | |||
+ | <FONT SIZE="2"><P>This part was received with the terminator ADH1 and we added it to all our constructs being fused with the pbs2 gene. | ||
+ | <UL TYPE="CIRCLE"> | ||
+ | <LI> yeast-sfGFP-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486026) | ||
+ | <LI> RLuc-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486027) | ||
+ | <LI> yeast-sfGFP_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486029) | ||
+ | <LI> RLuc_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486030) | ||
+ | |||
+ | </UL></P></FONT> | ||
+ | |||
+ | ===References=== | ||
+ | |||
+ | Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee et al (PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PubMed) | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K1486008 parameters</partinfo> |
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Revision as of 16:18, 27 September 2014
Yeast optimized superfolder GFP
Purpose of the Biobrick
This part encodes for the KanMx resistance gene and confers dominant selection of geneticin to yeast. We fused it to our constructs to select the transformants after homologous recombinations. It was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3.
Experiment 1:
Associated Biobricks
This part was received with the terminator ADH1 and we added it to all our constructs being fused with the pbs2 gene.
- yeast-sfGFP-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486026)
- RLuc-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486027)
- yeast-sfGFP_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486029)
- RLuc_N-ADH1-Kan ( https://parts.igem.org/Part:BBa_K1486030)
References
Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee et al (PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PubMed)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2213
Illegal PstI site found at 3446 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal PstI site found at 2213
Illegal PstI site found at 3446 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3657
Illegal BamHI site found at 1144
Illegal XhoI site found at 1283
Illegal XhoI site found at 2470 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2213
Illegal PstI site found at 3446 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2213
Illegal PstI site found at 3446
Illegal AgeI site found at 979
Illegal AgeI site found at 1694
Illegal AgeI site found at 2881 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961