Difference between revisions of "Part:BBa K1331001"
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RhlA together with RhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in ''Pseudomonas aeruginosa''.
 
RhlA together with RhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in ''Pseudomonas aeruginosa''.
 +
This part includes 59bp downstream noncoding gene of RhlA.
 
A mutation has been done to remove the PstI restriction site contained in the coding sequence, which won't change the amino acid sequence.
 
A mutation has been done to remove the PstI restriction site contained in the coding sequence, which won't change the amino acid sequence.
  

Revision as of 04:18, 26 September 2014

Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6

RhlA together with RhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. This part includes 59bp downstream noncoding gene of RhlA. A mutation has been done to remove the PstI restriction site contained in the coding sequence, which won't change the amino acid sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
    Illegal BamHI site found at 629
    Illegal XhoI site found at 805
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI.rc site found at 478