Difference between revisions of "Part:BBa K1365020:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Used primers:  
+
Used primers: <br>
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forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT-3'  
+
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT-3' <br>
−
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT-3'  
+
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT-3' <br>
−
to PCR sfGFP(Bs) from the plasmid and add the BioBrick prefix and suffix.  
+
to PCR sfGFP(Bs) from the plasmid and add the BioBrick prefix and suffix.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 20:09, 24 September 2014


sfGFP(Bs)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Design Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT-3'
to PCR sfGFP(Bs) from the plasmid and add the BioBrick prefix and suffix.

Source

PCR on plasmid MG_sfgfp(Bs), used in the study 'Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging', see references.

Plasmid kindly provided by the Molecular Genetics group of the Rijksuniversiteit Groningen.

References