Difference between revisions of "Part:BBa K1333201"

Revision as of 04:25, 24 September 2014

lambda cI-LGF2(D78A)

Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 712
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 767
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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Functional Parameters

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This is a graph of B2H system

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References:

name of the paper

family	with BBa_K1333200, BBa_K1333202, BBa_K1333203
function	hardly interact with Gal11P
protein	fusion protein of lambda cI and LGF2

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1333201 short</partinfo>
-Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78. As one of the components in Bacterial Two-hybrid System, it causes a less interaction with GAL11P compared to the normalized WT.
+Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.
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