Difference between revisions of "Part:BBa K1333201:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The LGF2 is mutated from GAC to GCC at site 78. | |
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===Source=== | ===Source=== | ||
Latest revision as of 04:19, 24 September 2014
lambda cI-LGF2(D78A)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 712
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 767
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The LGF2 is mutated from GAC to GCC at site 78.
Source
It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).