Difference between revisions of "Part:BBa K1365000:Design"
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===Source=== | ===Source=== | ||
| − | Obtained by PCR on the genome of L. lactis NZ9700, a nisin producing strain. | + | Obtained by PCR on the genome of ''L. lactis NZ9700'', a nisin producing strain. |
===References=== | ===References=== | ||
Revision as of 08:40, 23 September 2014
NisA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA-3'
to PCR NisA from the genome and add the BioBrick prefix and suffix.
Source
Obtained by PCR on the genome of L. lactis NZ9700, a nisin producing strain.