Difference between revisions of "Part:BBa K1491032"

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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1491031 short</partinfo>
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<partinfo>BBa_K1491032 short</partinfo>
  
 
T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell.
 
T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell.

Revision as of 16:22, 22 September 2014

T7 promoter-RBS-RFP-T7 Terminator

T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2950
    Illegal AgeI site found at 2914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2425