Difference between revisions of "Part:BBa K1491032"

 
(Just term -> Device)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
{|width='80%'
+
<partinfo>BBa_K1491031 short</partinfo>
−
|-
+
 
−
|width=35% valign='top'|
+
T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell.
−
[[Image:Terminator.png]]
+
−
<partinfo>BBa_K1491032 parameters</partinfo>
+
−
| width=50% valign='top' style='border: 1px solid black'|
+
−
<partinfo>BBa_K1491032 short</partinfo>
+
−
* Terminates transcription of plasmid by T7 RNA polymerase  
+
  
−
|}
+
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
  
−
'''Secondary Structure'''
+
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K1491031 SequenceAndFeatures</partinfo>
  
−
[[Image:Mfold-K1491032-1.png]]
 
  
−
<hr>
+
<!-- Uncomment this to enable Functional Parameter display
−
'''Measurement'''
+
===Functional Parameters===
−
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
+
<partinfo>BBa_K1491031 parameters</partinfo>
 +
<!-- -->

Revision as of 18:08, 19 September 2014

J23115-B0034-T7 RNA Polymerase with Dead ER Intein

T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2950
    Illegal AgeI site found at 2914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2425