Difference between revisions of "Part:BBa K1399015"
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The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. | The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. | ||
| + | This part can be considered as improved version of [[Part:BBa_J04431]] as it does not contain StuI restriction site coding for additional 'RP' residues between GFP and LVA tag. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 17:34, 19 September 2014
Plac-GFP(LVA)
Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
This part can be considered as improved version of Part:BBa_J04431 as it does not contain StuI restriction site coding for additional 'RP' residues between GFP and LVA tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870