Difference between revisions of "Part:BBa K1398007"

 
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<partinfo>BBa_K1398007 short</partinfo>
 
<partinfo>BBa_K1398007 short</partinfo>
  
 
A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008).
 
A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008).
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The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (also found in BBa_1398005).
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The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (BBa_K1398008). It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (BBa_K660004), a double STOP codon and a double terminator made up of BBa_B0010 and BBa_B0012.
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It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (BBa_B0015).
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Revision as of 10:04, 19 September 2014

NemR Promoter Reporter

A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008). The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (BBa_K1398008). It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (BBa_K660004), a double STOP codon and a double terminator made up of BBa_B0010 and BBa_B0012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]