Difference between revisions of "Part:BBa K1351000"
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Displayed on the surface of ''Bacillus subtilis'', it should enable ''B. subtilis'' to bind specifically to ''S. aureus''. | Displayed on the surface of ''Bacillus subtilis'', it should enable ''B. subtilis'' to bind specifically to ''S. aureus''. | ||
| − | This part was generated in RFC25 standard with the following | + | This part was generated in RFC25 standard with the following prefix and suffix: <br> |
| − | ENX_SD_Ngo_fwd: 5' | + | ENX_SD_Ngo_fwd: 5' '''GAATTC'''-'''GCGGCCGC'''-T-'''TCTAGA'''-GT-<u>AAGGAGG</u>-A-'''GCCGGC'''-ATG 3'<br> |
| − | PNS_Age_rev: 5' | + | PNS_Age_rev: 5' '''CTGCAG'''-'''CGGCCG <u>C'''-T-'''A</u> <u>CTA</u>GT'''-A<u>TTA</u>-'''ACCGGT''' 3' <br> |
| + | Restriction sites in bold, Shine-Dalgarno sequence and stop codons underlined. | ||
| + | |||
| + | |||
Revision as of 05:11, 18 September 2014
beta-Neurexin-derived peptide binding to S. aureus adhesin SdrC
Short peptide of 11 amino acids corresponding to the N-terminus of the human protein neurexin beta-1. It includes the binding site (HHIHHFH) for the Staphylococcus aureus adhesin SdrC. Displayed on the surface of Bacillus subtilis, it should enable B. subtilis to bind specifically to S. aureus.
This part was generated in RFC25 standard with the following prefix and suffix:
ENX_SD_Ngo_fwd: 5' GAATTC-GCGGCCGC-T-TCTAGA-GT-AAGGAGG-A-GCCGGC-ATG 3'
PNS_Age_rev: 5' CTGCAG-CGGCCG C-T-A CTAGT-ATTA-ACCGGT 3'
Restriction sites in bold, Shine-Dalgarno sequence and stop codons underlined.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]