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| | ===Applications of BBa_K1499500=== | | ===Applications of BBa_K1499500=== |
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| − | After ligating this construct together and into the BioBrick backbone, we transformed it into the NEB 5-alpha strain of E. coli. The colonies that fluoresced were most likely to have been successfully transformed, and thus these were sent off for sequencing. The sequencing data showed that our construct was correct (see below), so we were able to submit for sequencing).
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| − | [[File:lux sequencing data.jpg]]
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| − | Although our sequencing data was good, we realized that we had not induced the lac-repressible promoter, and thus the colonies should not have been fluorescing yet. After doing more research, we realized that the strain of E. coli we were using was not lactose deficient, thus the quorum sensing cascade was being activated by the natural presence of lactose. We ordered a lacI^q strain of E. coli, which are lac deficient, so that we could have complete control of GFP expression. However, when we performed the transformation of the construct into this strain, all of the colonies still fluoresced.
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| | ===User Reviews=== | | ===User Reviews=== |
Revision as of 01:29, 15 September 2014
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Applications of BBa_K1499500
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