Difference between revisions of "Part:BBa K1412924:Design"

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<partinfo>BBa_K1412924 short</partinfo>
 
<partinfo>BBa_K1412924 short</partinfo>

Revision as of 06:55, 9 September 2014

GFP generator with J23101


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


Design Notes

This part consists of Anderson promoter J23101 and GFP generator BBa_E0240. It can be used for characterization of promoter J23101. When in backbone vector pSB3K3, the copies is low, so we need observe it carefully. While in backbone vector pSB1C3, the expression is strong, so we can observe it easily.

Source

BBa_K82005 BBa_E0240

References

[1]Bagh, Sangram, Mahuya Mandal, and David R. McMillen. "Minimal genetic device with multiple tunable functions." Physical Review E 82.2 (2010): 021911. Available from: http://journals.aps.org/pre/abstract/10.1103/PhysRevE.82.021911.