Difference between revisions of "Part:BBa K1412829"
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<i>Plac-RBS(0.01)-CheZ-TT</i> | <i>Plac-RBS(0.01)-CheZ-TT</i> | ||
− | This part consists of a <i>CheZ</i> gene which can express CheZ protein deciding <i>E.coli</i> whether tumble or swim straight.In this light, we can characterize the RBS and promoter efficiency by just change different promoters or ribosome binding sites. | + | This part consists of a <i>CheZ</i> gene which can express CheZ protein deciding <i>E.coli</i> whether tumble or swim straight.In this light, we can characterize the RBS and promoter efficiency by just change different promoters or ribosome binding sites.Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of <i>CheZ</i>. |
3H:13-P3-3H BBa_R0010 | 3H:13-P3-3H BBa_R0010 |
Revision as of 06:50, 9 September 2014
Characterize efficiency of RBS with chemotaxis
Plac-RBS(0.01)-CheZ-TT This part consists of a CheZ gene which can express CheZ protein deciding E.coli whether tumble or swim straight.In this light, we can characterize the RBS and promoter efficiency by just change different promoters or ribosome binding sites.Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of CheZ.
3H:13-P3-3H BBa_R0010 2L:14-P2-2L BBa_B0033 18G:14-P1-18G BBa_K629003 4F:13-P3-4F BBa_B0015
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]