Difference between revisions of "Part:BBa K1412001"

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[[File:Curve_of_chemotaxis_diameter_over_time_under_different_Ara_concentration_.png |500px]]
 
[[File:Curve_of_chemotaxis_diameter_over_time_under_different_Ara_concentration_.png |500px]]
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== protocol ==
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'''Bold text'''
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1.Transformation
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2.Extract plasmids
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3.Digestion
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4.DNA gel electrophoresis
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5.Gel Extraction
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6.ligation
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7.Transformation
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8.Extract plasmids
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9.Digestion
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10.DNA gel electrophoresis
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11.Transform the plasmid into CL-1
  
 
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== '''Sequence and Features''' ==
 
== '''Sequence and Features''' ==
 
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Revision as of 04:49, 5 September 2014

Endow the E.coli engineered strain(CL-1) the ability of chemotaxis


What it is

Composite part enables the chemotaxis of our engineering bacteria could be regulated by IPTG and L-Arabinose; pBAD and pLac promoters control the expression of Lac I and CheZ.


What it does

when L-Arabinose is added, pBAD promoter is activated, expressing Lac I, inhibiting the expression of CheZ. When IPTG is added, IPTG combines with Lac I, forming a complex, thus relief the inhibition and the CheZ express, make the ability of chemotaxis recover.


How to use it in their project

Use IPTG as an inducer and L-Arabinose as an inhibitor to take control of the chemotaxis of the engineering bacteria.


Experimental data

Curve of chemotaxis diameter under different antibiotics concentrations.png


Curve of chemotaxis diameter over time under different IPTG concentration .png


Curve of chemotaxis diameter over time under different Ara concentration .png


protocol

Bold text 1.Transformation 2.Extract plasmids 3.Digestion 4.DNA gel electrophoresis 5.Gel Extraction 6.ligation 7.Transformation 8.Extract plasmids 9.Digestion 10.DNA gel electrophoresis 11.Transform the plasmid into CL-1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]