Difference between revisions of "Part:BBa K1442024:Design"

(→‎References)
(→‎Design Notes)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
None.  
+
Biobrick compatible.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 13:47, 4 September 2014

NKRF IRES


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 340


Design Notes

Biobrick compatible.

Source

NF-kappa-B-repressing factor (NKRF) is derived from a human source, the IRES was therefore derived from a human gene.

References

http://iresite.org/IRESite_web.php?page=view&entry_id=140

[1] Bochkov and Palmenberg, 2006, Translational efficiency of EMCV IRES in bicistronic vectors is dependent upon IRES sequence and gene location, BioTechniques, 41:283-292 (September 2006) http://www.biotechniques.com/multimedia/archive/00002/BTN_A_000112243_O_2391a.pdf