Difference between revisions of "Part:BBa K1346013"
Line 5: | Line 5: | ||
[[File:PCL2-zoomed.png|900px]] | [[File:PCL2-zoomed.png|900px]] | ||
− | Compared to the other alpha factor responsive promoters, | + | Compared to the other alpha factor responsive promoters, pPCL2 has a very high basal expression level and the second highest maximum output, with only pAGA1 having a higher expression level. |
[[File:140722_AFRP_GFP_DB_ROUND_4_zoomed.png|900px]] | [[File:140722_AFRP_GFP_DB_ROUND_4_zoomed.png|900px]] |
Latest revision as of 21:47, 3 September 2014
pPCL2
Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 7 concentrations of alpha factor.
Compared to the other alpha factor responsive promoters, pPCL2 has a very high basal expression level and the second highest maximum output, with only pAGA1 having a higher expression level.
Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 780
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 835