Difference between revisions of "Part:BBa K1346010"
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[[File:CLG1-zoomed.png|900px]] | [[File:CLG1-zoomed.png|900px]] | ||
| − | Compared to the other alpha factor responsive promoters, pCLG1 has a high basal expression level, a very broad range of expression, and a mid-level maximum output, although the curve suggests that the expression level had not yet been maximized | + | Compared to the other alpha factor responsive promoters, pCLG1 has a high basal expression level, a very broad range of expression, and a mid-level maximum output, although the curve suggests that the expression level had not yet been maximized and pCLG1 is not very responsive to alpha factor. |
[[File:140722_AFRP_GFP_DB_ROUND_4_zoomed.png|900px]] | [[File:140722_AFRP_GFP_DB_ROUND_4_zoomed.png|900px]] | ||
Latest revision as of 21:24, 3 September 2014
pCLG1
Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 7 concentrations of alpha factor.
Compared to the other alpha factor responsive promoters, pCLG1 has a high basal expression level, a very broad range of expression, and a mid-level maximum output, although the curve suggests that the expression level had not yet been maximized and pCLG1 is not very responsive to alpha factor.
Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]