Difference between revisions of "Part:BBa K1319001"
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This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP). | This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP). | ||
Revision as of 19:36, 3 September 2014
RFC[25]-compatible dark quencher based on K1319000 (E0030)
This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP).
Two mutations were introduced that eliminated fluorescence:
- L90I
- Y145W
References
- Ganesan S, Ameer-Beg SM, Ng TT, Vojnovic B, Wouters FS. A dark yellow
fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP. Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4089-94. Epub 2006 Mar 6. PubMed PMID: 16537489; PubMed Central PMCID: PMC1449651. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449651/?report=classic PubMed Central]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]