Difference between revisions of "Part:BBa K1412002"
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== What it is == | == What it is == | ||
| − | + | Compared to part BBa_1412001, this part substitute BBa_R0040 for BBa_K206000, a constant expression promoter in our engineered bacteria CL-1. | |
== What it does == | == What it does == | ||
| − | + | As a control group for K1412001,we use this part toEliminate background expression of AraC, and it’s also capable to form ellipse on the plate under the induction of IPTG. | |
== How to use it == | == How to use it == | ||
| − | + | Transforming this part into CL-1,we get the engineering bacteria. Incubate on the plate with fit concentration of IPTG and chloromycetin, we observed the chemotaxis diameter over time, and get the experimental data. It is also used to form ellipse. | |
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Revision as of 06:23, 2 September 2014
Control group of K1412001
What it is
Compared to part BBa_1412001, this part substitute BBa_R0040 for BBa_K206000, a constant expression promoter in our engineered bacteria CL-1.
What it does
As a control group for K1412001,we use this part toEliminate background expression of AraC, and it’s also capable to form ellipse on the plate under the induction of IPTG.
How to use it
Transforming this part into CL-1,we get the engineering bacteria. Incubate on the plate with fit concentration of IPTG and chloromycetin, we observed the chemotaxis diameter over time, and get the experimental data. It is also used to form ellipse.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]