Difference between revisions of "Part:BBa K1412001"
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Use IPTG as an inducer and L-Arabinose as an inhibitor to take control of the chemotaxis of the engineering bacteria. | Use IPTG as an inducer and L-Arabinose as an inhibitor to take control of the chemotaxis of the engineering bacteria. | ||
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| + | == '''Experimental data''' == | ||
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| + | [[File:Curve_of_chemotaxis_diameter_over_time_under_different_IPTG_concentration_.png]] | ||
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| + | [[File:Curve_of_chemotaxis_diameter_under_different_antibiotics_concentrations.png ]] | ||
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| + | [[File:Curve_of_chemotaxis_diameter_over_time_under_different_Ara_concentration_.png ]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 15:55, 1 September 2014
Endow the E.coli engineered strain(CL-1) the ability of chemotaxis
What it is
Composite part enables the chemotaxis of our engineering bacteria could be regulated by IPTG and L-Arabinose; pBAD and pLac promoters control the expression of Lac I and CheZ.
What it does
when L-Arabinose is added, pBAD promoter is activated, expressing Lac I, inhibiting the expression of CheZ. When IPTG is added, IPTG combines with Lac I, forming a complex, thus relief the inhibition and the CheZ express, make the ability of chemotaxis recover.
How to use it in their project
Use IPTG as an inducer and L-Arabinose as an inhibitor to take control of the chemotaxis of the engineering bacteria.
Experimental data
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]