Difference between revisions of "Part:BBa K1460003:Design"

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===Source===
 
===Source===
  
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Anderson promoter is that of part BBa_J23102.  Terminator is that of part BBa_B1006.  NixA gene is naturally found in H. pylori.  This part was synthesized by GenScript in the vector pUC57.  Part was then transferred into pSB1C3 using standard cloning procedures.  
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Anderson promoter is that of part BBa_J23102.  Terminator is that of part BBa_B1006.  NixA gene is naturally found in <i>H. pylori</i>.<sup>[1]</sup> This part was synthesized by GenScript in the vector pUC57.  Part was then transferred into pSB1C3 using standard cloning procedures.
  
 
===References===
 
===References===
  
 
[1] Mobley, H., Garner, R., & Bauerfeind, P. (1995). <i>Helicobacter pylori</i> nickel-transport gene nixA: Synthesis of catalytically active urease in <i>Escherichia coli</i> independent of growth conditions. <i>Molecular Microbiology</i>, 97-109.
 
[1] Mobley, H., Garner, R., & Bauerfeind, P. (1995). <i>Helicobacter pylori</i> nickel-transport gene nixA: Synthesis of catalytically active urease in <i>Escherichia coli</i> independent of growth conditions. <i>Molecular Microbiology</i>, 97-109.

Latest revision as of 16:01, 26 August 2014


Anderson Promoter + NixA + Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Anderson promoter provides strong constitutive expression of NixA in E. coli.


Source

Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in H. pylori.[1] This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures.

References

[1] Mobley, H., Garner, R., & Bauerfeind, P. (1995). Helicobacter pylori nickel-transport gene nixA: Synthesis of catalytically active urease in Escherichia coli independent of growth conditions. Molecular Microbiology, 97-109.