Difference between revisions of "Part:BBa K1460004:Design"

 
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===Source===
 
===Source===
  
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Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006.  merT/merP genes are naturally found in Pseudomonas aeruginosa.  This part was synthesized by GenScript in the vector pUC57.  The part was then transferred into pSB1C3 using standard cloning procedures.
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Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006.  merT/merP genes are naturally found in <i>Pseudomonas aeruginosa</i>.<sup>[1]</sup> This part was synthesized by GenScript in the vector pUC57.  The part was then transferred into pSB1C3 using standard cloning procedures.
  
 
===References===
 
===References===

Revision as of 15:54, 26 August 2014


Anderson Promoter + MerT + MerP + ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 92


Design Notes

The Anderson promoter provides strong constitutive expression of merT/merP in E. coli


Source

Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. merT/merP genes are naturally found in Pseudomonas aeruginosa.[1] This part was synthesized by GenScript in the vector pUC57. The part was then transferred into pSB1C3 using standard cloning procedures.

References