Difference between revisions of "Part:BBa K1346003"
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Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7. | Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7. | ||
| − | **Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)* | + | * *Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)* |
[[File:140630_pTEF1_GFP_ROUND_1.png|900px]] | [[File:140630_pTEF1_GFP_ROUND_1.png|900px]] | ||
Revision as of 21:20, 5 August 2014
pTEF1(m10) - constitutive promoter
Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7.
- *Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)*
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 169