Difference between revisions of "Part:BBa K1346000"

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<partinfo>BBa_K1346000 short</partinfo>
 
<partinfo>BBa_K1346000 short</partinfo>
  
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Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than M7.
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Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than M7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.
  
 
[[File:140630 pTEF1 GFP ROUND 1.png|900px]]
 
[[File:140630 pTEF1 GFP ROUND 1.png|900px]]
  
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data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:00, 5 August 2014

pTEF1(m3) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than M7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.

140630 pTEF1 GFP ROUND 1.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169