Difference between revisions of "Part:BBa J119363"
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<partinfo>BBa_J119363 short</partinfo> | <partinfo>BBa_J119363 short</partinfo> | ||
| − | The construct is a fitness module for ammeline. The P5 promoter drives a mutated riboswitch (N2) that allows translation in the presence of ammeline (made by demethylation of melamine) of a TetA fitness gene. | + | The construct is a fitness module for ammeline. The P5 promoter drives a mutated riboswitch (N2 with mutations T85A, T89A, G93C, T94C, G95T, T96G) that allows translation in the presence of ammeline (made by demethylation of melamine) of a TetA fitness gene. |
Latest revision as of 19:55, 22 July 2014
Ammeline Fitness Module with Riboswitch N2 + TetA (T85A, T89A, G93C, T94C, G95T, T96G)
The construct is a fitness module for ammeline. The P5 promoter drives a mutated riboswitch (N2 with mutations T85A, T89A, G93C, T94C, G95T, T96G) that allows translation in the presence of ammeline (made by demethylation of melamine) of a TetA fitness gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 334
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 480
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 506
Illegal NgoMIV site found at 874
Illegal NgoMIV site found at 1034 - 1000COMPATIBLE WITH RFC[1000]