Difference between revisions of "Part:BBa J176020:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J176020 short</partinfo>
 
<partinfo>BBa_J176020 short</partinfo>
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===Design Notes===
 
===Design Notes===
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TBA
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* Gel-shift characterization: The the stoichiometry of the 1-147 N-terminal portion of Gal4 is Gal4DB:UAS17-mer = 2:1. [1]. The 17-mer is 5'-CGGAAGACTCTCCTCCG.
  
  
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===References===
 
===References===
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1. Carey, M., Kakidani, H., Leatherwood, J., Mostashari, F., & Ptashne, M. (1989). An amino-terminal fragment of GAL4 binds DNA as a dimer. Journal of Molecular Biology, 209(3), 423–432.

Revision as of 02:01, 22 July 2014

Gal4 DB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137


Design Notes

  • Gel-shift characterization: The the stoichiometry of the 1-147 N-terminal portion of Gal4 is Gal4DB:UAS17-mer = 2:1. [1]. The 17-mer is 5'-CGGAAGACTCTCCTCCG.


Source

TBA

References

1. Carey, M., Kakidani, H., Leatherwood, J., Mostashari, F., & Ptashne, M. (1989). An amino-terminal fragment of GAL4 binds DNA as a dimer. Journal of Molecular Biology, 209(3), 423–432.