Difference between revisions of "Part:BBa K1346002:Design"

 
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===References===
 
===References===
 +
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Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae
 +
Elke Nevoigt1,2, Jessica Kohnke2, Curt R. Fischer1, Hal Alper1, Ulf Stahl2 and Gregory Stephanopoulos1,*
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http://aem.asm.org/content/72/8/5266.long

Latest revision as of 21:26, 15 July 2014

pTEF1(m7) - constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169


Design Notes

m7 has a difference of about 25bp from pTEF1


Source

Mutant of pTEF1 which is endogenous to the S. Cerevisiae genome.

References

Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae Elke Nevoigt1,2, Jessica Kohnke2, Curt R. Fischer1, Hal Alper1, Ulf Stahl2 and Gregory Stephanopoulos1,*

http://aem.asm.org/content/72/8/5266.long