Difference between revisions of "Part:BBa K1346001:Design"

m
m (→‎Design Notes)
 
Line 8: Line 8:
 
mutant of pTEF1
 
mutant of pTEF1
  
−
 
+
approximately 8 bp of differences.
  
 
===Source===
 
===Source===

Latest revision as of 19:09, 15 July 2014

pTEF1(m6) - constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 168


Design Notes

mutant of pTEF1

approximately 8 bp of differences.

Source

Mutant of pTEF1 which is endogenous to the s. cerevisiae genome. Actual mutant found in http://aem.asm.org/content/72/8/5266.long - created by authors.

References

Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae Elke Nevoigt1,2, Jessica Kohnke2, Curt R. Fischer1, Hal Alper1, Ulf Stahl2 and Gregory Stephanopoulos1,*

http://aem.asm.org/content/72/8/5266.long