Difference between revisions of "Part:BBa J100163"
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<partinfo>BBa_J100163 short</partinfo> | <partinfo>BBa_J100163 short</partinfo> | ||
| − | This has a two mutations from the original riboswitch sequence found in part J119332. This version is designed to be a more effective riboswitch. | + | This has a two mutations from the original riboswitch sequence found in part J119332. This version is designed to be a more effective riboswitch. As with part J119332, base 431 is mutated from an A (tyrosine) to a G (cysteine) in the RFP CDS. |
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Latest revision as of 20:09, 9 July 2014
Strong Promoter + M6-R10 Ammeline Riboswitch + RFP
This has a two mutations from the original riboswitch sequence found in part J119332. This version is designed to be a more effective riboswitch. As with part J119332, base 431 is mutated from an A (tyrosine) to a G (cysteine) in the RFP CDS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 741
Illegal AgeI site found at 853 - 1000COMPATIBLE WITH RFC[1000]