Difference between revisions of "Part:BBa K1033926"

Revision as of 13:04, 12 June 2014

asPink, pink chromoprotein (incl RBS, J23110)

This chromoprotein from the coral Anemonia sulcata, asPink (also known as asCP or asFP595), naturally exhibits strong color when expressed. The protein has an absorption maximum at 572 nm giving it a pink/purple color visible to the naked eye. The strong color is readily observed in both LB or on agar plates after less than 24 hours of incubation. The protein asPink has significant sequence homologies with proteins in the GFP family.

Usage and Biology

This part is useful as a reporter.

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iGEM2013 Uppsala: Expression of asPink in E. coli DH5alpha by promoter J23110 from medium copy plasmid pSB3K3 (left) or high copy plasmid pSB1K3 (right).

Source

The protein was first extracted and characterized by Lukyanov et. al. 2000 under the name asFP595 (GenBank: AAG02385.1). This version is codon optimized for E coli by Genscript.

References

[http://www.jbc.org/content/275/34/25879.short]Lukyanov, Konstantin A., et al. "Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog." Journal of Biological Chemistry 275.34 (2000): 25879-25882.

[http://www.jbc.org/content/280/4/2401.short]Wilmann, Pascal G., et al. "Variations on the GFP Chromophore - A Polypeptide Fragmentation Within The Chromophore Revealed In The 2.1-Å Crystal Structure Of A Nonfluorescent Chromoprotein From Anemonia Sulcata." Journal of Biological Chemistry 280.4 (2005): 2401-2404.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 This part is useful as a reporter.
-[[Image:Uppsala2013_chromoproteins.JPG|900px]]
+[[Image:AsPink.jpg|700px]]
-'''iGEM13_Uppsala:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing meffRed <partinfo>BBa_K1033922</partinfo>, aeBlue <partinfo>BBa_K1033929</partinfo>, tsPurple <partinfo>BBa_K1033905</partinfo>, asPink (asCP) <partinfo>BBa_K1033927</partinfo>, fwYellow <partinfo>BBa_K1033910</partinfo>, meffBlue <partinfo>BBa_K1033902</partinfo>, meffRed <partinfo>BBa_K1033922</partinfo> and scOrange <partinfo>BBa_K1033913</partinfo>.
+'''iGEM2013 Uppsala:'''  Expression of asPink in ''E. coli'' DH5alpha by promoter J23110 from medium copy plasmid pSB3K3 (left) or high copy plasmid pSB1K3 (right).
 ===Source===