Difference between revisions of "Part:BBa K1256004"

Revision as of 01:59, 10 June 2014

pSB1C3-Plac-SS-Cecropin-MprF

MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) using NheI and BamHI sites. Next, the cds of cecropin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The amino acid sequences of cecropin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the [http://sg.idtdna.com/CodonOpt codon optimization] tool provided by Integrated DNA Technologies (IDT).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 398
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2924
Illegal BamHI site found at 2993
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 371
Illegal NgoMIV site found at 1319
Illegal NgoMIV site found at 2375
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1256004 short</partinfo>
-TEST
+[[File: Mingdao_pSB1C3-Plac-SS-Cecropin-MprF.png|400px]]
+MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of [https://parts.igem.org/Part:BBa_K1256003 pSB1C3-Plac-SS-NB (Part:BBa_K1256003)] using NheI and BamHI sites. Next, the cds of cecropin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The amino acid sequences of cecropin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the [http://sg.idtdna.com/CodonOpt codon optimization] tool provided by Integrated DNA Technologies (IDT).
 <!-- Add more about the biology of this part here