Difference between revisions of "Part:BBa K1256002"
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[[File:Mingdao_pSB1C3-Plac-NB-Cm-MN.png|400px]]
 
[[File:Mingdao_pSB1C3-Plac-NB-Cm-MN.png|400px]]
  
−
Follow the design of [https://parts.igem.org/Part:BBa_K1256001 BBa_K1256001]
+
Follow the design of [https://parts.igem.org/Part:BBa_K1256001 BBa_K1256001], more restriction enzyme sites were introduced onto the vector of pSB1C3. MfeI and NsiI sites were generated following the Cm resistance gene cassette and can be used to ligate the BioBrick parts with EcoRI and PstI. Coding regions cloning in this region can be driven by the Cm promoter. This design is benefit for multiple parts assembly.
  
  

Revision as of 15:39, 9 June 2014

pSB1C3-Plac-NB-Cm-MN

Mingdao pSB1C3-Plac-NB-Cm-MN.png

Follow the design of BBa_K1256001, more restriction enzyme sites were introduced onto the vector of pSB1C3. MfeI and NsiI sites were generated following the Cm resistance gene cassette and can be used to ligate the BioBrick parts with EcoRI and PstI. Coding regions cloning in this region can be driven by the Cm promoter. This design is benefit for multiple parts assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found at 1076
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3145
    Illegal NheI site found at 221
    Illegal SpeI site found at 1077
    Illegal PstI site found at 1091
    Illegal NotI site found at 1084
    Illegal NotI site found at 2228
    Illegal NotI site found at 3151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3145
    Illegal BamHI site found at 933
    Illegal XhoI site found at 2108
    Illegal XhoI site found at 3021
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3145
    Illegal suffix found at 1077
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3145
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3160
    Illegal SpeI site found at 1077
    Illegal PstI site found at 1091
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.