Difference between revisions of "Part:BBa K177029"
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Latest revision as of 14:56, 19 May 2014
Safety Flag
The iGEM Safety and Security Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety [AT] igem [DOT] org with any questions.
Reason: Listeriolysin and Invasin parts
If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2021 Safety Page for more information.
cell Invasion device (testing)
makes E. coli a good bioweapon by allowing bacteria to enter and live inside human cells
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4093
Illegal XbaI site found at 2080
Illegal SpeI site found at 1894 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4093
Illegal NheI site found at 1607
Illegal NheI site found at 2269
Illegal SpeI site found at 1894 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4093
Illegal BglII site found at 2077 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4093
Illegal XbaI site found at 2080
Illegal SpeI site found at 1894 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4093
Illegal XbaI site found at 2080
Illegal SpeI site found at 1894 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2065