Difference between revisions of "Part:BBa K177005"
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Latest revision as of 14:51, 19 May 2014
Safety Flag
The iGEM Safety and Security Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety [AT] igem [DOT] org with any questions.
Reason: Listeriolysin and Invasin parts
If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2021 Safety Page for more information.
Bistable_1
Bistable switch for lysteriolysin/invasin expression control
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6521
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2207
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3080
Illegal NgoMIV site found at 7425
Illegal AgeI site found at 2589
Illegal AgeI site found at 2913
Illegal AgeI site found at 3048
Illegal AgeI site found at 3126
Illegal AgeI site found at 4635
Illegal AgeI site found at 5139
Illegal AgeI site found at 8273 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 9093