Difference between revisions of "Part:BBa K225001"

Latest revision as of 15:45, 5 May 2014

Green light activated actuator

Green light activated actuator composed of the phycocyanobilin synthesis genes (ho1 and pcyA) and the green light receptor (GlrN).

Usage and Biology

GlrN is the fusion protein of light responsive domains of CcaS and EnvZ designed by changing the histidine kinase region of CcaS to EnvZ domain of cph8 (BBa_I15010).

Requires phycocyanobilin biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation.
Must be used in E. coli deficient in natural EnvZ.

Expected function (without experimental proof)

Exposure to green light (535 nm) phosphorylate EnvZ histidin kinase domain. The activated EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpR positive promoter (BBa_R0083).

NOTE. This part may be thought of as a sensor for green light, not an actuator [D.Endy, 5 May 2014]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2202
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1564
Illegal BglII site found at 2456
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1117
1000
COMPATIBLE WITH RFC[1000]

@@ Line 15: / Line 15: @@
 Exposure to green light (535 nm) phosphorylate EnvZ histidin kinase domain. The activated EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpR positive promoter (BBa_R0083).
-NOTE.  This part may be thought of as a sensor for green light, not an actuator [D.Endy, 5 May 2014]
+'''NOTE.'''  This part may be thought of as a sensor for green light, not an actuator [D.Endy, 5 May 2014]
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