Difference between revisions of "Part:BBa M36596"

 
(Registry curation)
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_M36596 short</partinfo>
 
<partinfo>BBa_M36596 short</partinfo>
Line 15: Line 14:
 
<partinfo>BBa_M36596 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_M36596 SequenceAndFeatures</partinfo>
  
 +
<DIV class='curation y2014'>
 +
Circular specification. Registry curation 5-4-2014
 +
 +
This part was defined as a composite part M36595 - Itself - M36281.
 +
This definition is recursive and not valid. The designer should define a basic
 +
part with only the parts sequence and then define a separate composite part
 +
with the enclosing parts.
 +
 +
We have redefined it as a basic part.
 +
</div>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 21:30, 4 May 2014

Oxygen-tolerant hydrogenase (with RBS and terminator)

Hydrogenase is an enzyme that catalyzes the reversible oxidation of H2. This part encodes a hydrogenase actuator taken from the large subunit of the marine bacterium Hydrogenovibrius Marinus, which has been shown to be relatively tolerant to oxygen (Kim et. Al, 2011). This part consists of a strong bicistronic design RBS (found under BioFAB ID: pFAB1655), a codon-optimized HmMBH actuator gene taken from the sequence of the large subunit of hydrogenase found in hydrogenovibrius marinus (original sequence found under F2Z6J6 at uniprot.org), and a high efficiency transcriptional terminator (found under BBa_M36281 in the Registry of Standard Biological Parts). The mechanism of the part is relatively straightforward: Once induced with the PoPS signal, the actuator will initiate protein translation and express the hydrogenase enzyme. Our actuator was used in conjunction with a rhamnose sensor (e.g. BBa_M36284).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 433
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 433
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1101
    Illegal BamHI site found at 1428
    Illegal BamHI site found at 1605
    Illegal XhoI site found at 715
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 433
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 433
  • 1000
    COMPATIBLE WITH RFC[1000]

Circular specification. Registry curation 5-4-2014

This part was defined as a composite part M36595 - Itself - M36281. This definition is recursive and not valid. The designer should define a basic part with only the parts sequence and then define a separate composite part with the enclosing parts.

We have redefined it as a basic part.